Highly Resistant enterobacteriaceae and extended Spectrum Beta lactamase producing Klebsiella pneumoniae from clinical isolate within Bauchi metropolisName : Dr. Nazeef Idris Usman
Affliation : Assistant Professor
University : Bauchi State University, Gadau
Country : Nigeria
The Enterobacteriaceae are a large family of gram-negative bacteria that are facultative anaerobes, fermenting sugars to produce lactic acid and various other end products. Most also reduce nitrate to nitrite their characteristics have made them potential pathogens and easier to identified. Most prevalent among this family are E.coli and klebsiella pneumonia, recently these species developed resistant to many antibiotics with few exception among which include Beta lactams antibiotics. This study exploit the susceptibility profile of enterobacteriaceae to antibiotics and detection of Extended Spectrum Betalactameses (ESBL’s) producing klebsiella pneumoniae from clinical isolate. A total of 485 enterobacteriaceae isolate (E.coli, klebsiella pneumoniae, Enterobacter species, Proteus species, Salmonella typhi and Shigella species) mostly from Urine, High Virginal Swab (HVS), Endocervical Swab (ECS), Sputum and Blood where collected from selected hospital within Bauchi metropolis from April to August 2016 to study the Highly Resistant Enterobacteriaceae (HRE) and some ESBL producing Klebsiella pneumoniae among clinical isolates in hospital setting. Isolates were primarily screened for possible ESBL production and were phenotypically confirmed. Following Kirby Bauer disk diffusion method base on Clinical and Laboratory Standard Institute (CLSI) guidelines, Antibiotic susceptibility testing (AST) was further conducted. Among the 485 isolate isolates processed, 347(71.5%) were tested for MDR while 138(28.5%) klebsiella pneumoniea were tested for ESBL production. 56(40.6%) of klebsiella pneumoniae are ESBL producers while 82(59.4%) are non ESBL Producers. The phenotypic confirmatory test justifies the presence of ESBL Producers among the Klebsiella pneumoniae with some enterobacteriaceae that are MDR. There is also a need for further research to identify the effect of infection with ESBL producing bacteria within the study area.
Nazeef Idris Usman Attends Federal Polytechnic Bauchi Staff School And Birshi Primary School And Proceeded To Government Day Secondary School Army Barrack Later Government Science College Toro. He Graduated From Abubakar Tafawa Balewa University Bauchi Where He Studies Applied Microbiology And MSc. Medical Microbiology, And Was Opportune To Published Articles Relevant To His Field In Many Journals. Currently Working With Microbiology Department Of Bauchi State University Gadau