An insight on advances BioLumix Rapid Microbiology new methodology system in microbial infectious diseases, eradication of important pathogens and prevention of human diseases by developing new strategies towards medical products and problems.Name : Dr. Sherajuli Shelley
Affliation : Director
University : Nutra solutions
Country : USA
The BioLumix system is based on the utilization of chromogenic and fluorogenic substrates for the detection of activity of specific enzymes. Change in colors of a pH. indicator during microbial activity. Bromo cresol purple used as an indicator between 6-8 purple and pH 5.2 yellow, in Enterobacteriaceae bile -tolerant gram-negative bacteria and coliform media. Phenol red is on of the indicators in the staphylococcus medium. The hydrolysis of synthetic enzymes by bacterial enzymes causes an increase in fluence. Florigenic synthetic enzyme substrate containing 4-methyl umbelliferon are most commonly used. The e-coli assays employees 4-methyl umbelliferyl. Beta glucuronide (MUG). The enzyme beta glucuronide (GUD) present in 97% of e-coli strains can hydrolyze the colorless substrate MUG to yield a bluish fluorogenic product of 4- methylethylketone MU that fluoresces under UV light 366 nm of 4 -methylumbelliferone MU that fluoresces under UV LIGHT 366 NM present in the instrument. Regarding the carbon di oxide sensor: carbon dioxide CO2 is a universal metabolic produced by all microorganisms for aerobic microorganisms, the Krebs cycle is part of a metabolic pathway involved in the chemical conversion of carbohydrate, fats, and proteins into CO2, water and energy, and as a result all aerobic organisms generate CO2. The sensor is composed of a matrix of a polymeric material which is transparent to light and contains an indicator reagent sensitive to CO2 gas generated by the microorganisms. the matrix is configured to facilitate penetration of external light from LED and monitoring the change of color of the indicator from blue green to yellow due to CO2 production. A crucial element of the technology is the creation of two zones in each vial.an incubation zone upper part for the sample and microorganisms. this zone tends to contain product debris and turbidity due to microbial growth. A reading zone bottom part that remains optically clear. This two -zones vial eliminate interference of the optical pathway by the product and microbial turbidity. Since changes of color or fluorescence are monitored in the reading zone, results are not influenced by the presence of the sample or the growing microorganisms.