Prevalence of Carbapenem Resistant Gram Negative Esbls Producers in Wound InfectionsName : TOMILOLA ADESINA
Affliation : Professor
University : COVENANT UNIVERSITY
Country : Nigeria
The rapidly changing resistance pattern of multidrug resistant Gram-negative bacilli over the last decade is challenging. The spread of carbapenem resistant and ESBL producing organisms are by far the most current clinical issue in antibiotic resistance, globally. This study was carried out to determine the prevalence of carbapenem resistant Gram negative producing extended spectrum β-lactamases (ESBLs).
A total of forty nine (49) wound samples were obtained and cultured aseptically. Antibiogram studies were carried out, followed by the detection of ESBL production using double disk synergistic test.
The most prevalent organisms were Proteus sp. (39), Klebsiella sp. (15), P. aeruginosa (12), E. coli (10), S. marscencens (9), Citrobacter sp. (9), Enterobacter sp. (5) and Edwardsiella sp. (2). All organisms were resistant to a minimum of 4 different classes of antibiotics, while about twenty (20) were resistant to all antibiotics. A total of forty eight (47%) isolates were ESBL producers. All ESBL producers were resistant to at least one type of carbapenem antibiotics; all forty eight (100%) to meropenem, forty six (96%) to imipenem and thirty-seven (77%) to etarpenem antibiotics.
Carbapenemase and ESBL producers remain threats to public health and a risk factor for difficult to treat infections. There is need for further studies on mechanisms of ESBL and carbapenemase production, inclusion of ESBL and carbapenemase detection into routine sensitivity testing, review of empirical guide in antibiotics therapy within healthcare setting and control of cross transmission of carbapenem resistant and ESBL producing Gram negative bacilli.
Adesina, D. T., is a PhD candidate at Covenant university. Her research focuses on molecular characterization of Gram negative bacilli, producing plasmid mediated beta-lactamases from long term wound infections. She has actively engaged severally in the use of molecular biology methods such as Polymerase chain reaction (PCR), Gel electrophoresis, DNA extraction, plasmid curing, cloning and editing and analysis of gene sequences. She is currently supervised by Professor De and Professor Nwinyi from Covenant University.